Enzymatic characterization of 17 L-arabinose negative mutants of Escherichia coli.

Direct selection of L-arabinose negative mutants of Escherichia coli strain B@rl.

ROSS and ENGLESBERG (1959), using three and four factor transduction exG periments with phage PI bt, found that the mutational sites of 17 L-arabinose nonutilizing mutants of Escherichia coli were arranged in a linear sequence. The 17 mutants were divided into three distinct functional groups on the basis of biochemical analyses. Complete correspondence was found between the order of the mutati...

Using L-arabinose for Production of Human Growth Hormone in Escherichia coli, Studying the Processing of gIII::hGH Precursor

L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli.

Englesberg, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. Anderson, R. Weinberg, N. Lee, P. Hoffee, G. Huttenhauer, and H. Boyer. l-Arabinose-sensitive, l-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137-146. 1962-l-Arabinose-negative mutants of Escherichia coli B/r, ara-53 and ara-139, are deficient in the enzyme l-ribulose 5-phosphate 4-epime...

L-arabinose Negative Mutants of the L-ribulokinase Structural Gene Affecting the Levels of L-arabinose Isomerase in Escherichia Coli.

C @ 51 37 19 12 5 42 3 49 57 21 59 1.U 1 1 1 1 l 1 1 1 WENTY-two L-arabinose nonutilizing mutants of Eschrichia coli B/r have Tbeen ordered with regard to each other and a closely linked leucine marker by three-point tests using transduction of E . coli by phage Plbt (GROSS and ENGLESBERG 1959; ENGLESBERG, ANDERSON, WEINBERG, LEE, HOFFEE, HUTTENHAUER and BOYER 1962; BOYER, ENGLESBERG and WEINBE...

"In vivo" detection of L-arabinose-binding protein, CRM-negative mutants.

An "in vivo" assay for the detection of mutants negative to CRM (cross-reacting material) is described. l-Arabinose-negative mutants of Escherichia coli B/r were grown on Casamino Acids-l-arabinose plates to which a 3-ml agar layer, containing antiserum to the l-arabinose-binding protein (ABP), had been applied. After incubation and partial lysis of the clones "in situ," the plates were refrige...